|
KMID : 0368419970400010001
|
|
Journal of Plant Biology
1997 Volume.40 No. 1 p.1 ~ p.8
|
|
|
Partial Sequence Analysis of Randomly Selected Watermelon [Citrullus lanantus(Thunb.) Mansf.] cDNA Clones
|
|
Ok, Sung Han
Shin, Jeong Sheop/Chung, Young Soo/Kwon, Hyok Ji
|
|
|
Abstract
|
|
|
|
An expressed sequence tag (EST) is simply a segment of a sequence over 150 bp from a randomly selected cDNA. EST helps to quickly identify functions of expressed genes and to understand the complexity of gene expression with database comparison. Sequencing of random cDNA clones can be applicable to discovery of new genes, mapping of the genome, identification of coding regions in genomic sequences, and antisense method. To accomplish these goals, in this research, randomly selected cDNA sequencing was performed with watermelon plant. Among 30 clones picked up and analyzed, all clones had an insert length over 0.5 kb. The average size of insert was about 1.3 kb. The size range of most cDNA insert was 1.0-2.0 kb. For sequence comparison, data from the coding region at 5´ end of selected cDNA should be much more informative than those from the untranslated 3´ tail. Thirty clones were sequenced from one end (5´ end). Of these, 29 had no poly (A) tail in this direction, while only one was inverted. Thus, this library is over 96% unidirectional. Two clones had homologies to ribulose bisphosphate carboxylase/oxigenase (Rubisco) small subunit precursor gene. Thirteen cDNAs had high degree of sequence similarity to genes from other organisms. The remaining cDNA clones seem to be new genes not only in watermelon but also in all organisms.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|